Fig. 2. Localisation of CLIP-190 to unattached kinetochores. S2 cells were immunostained without (A,C) or after (B) incubation with colchicine. The right panels show magnified images of the regions marked with squares in the left panels. (A) Kinetochore localisation of CLIP-190. Staining of CLIP-190 overlaps with the one of BubR1, a known kinetochore protein. (B) Microtubule-independent localisation of CLIP-190 to kinetochores. CLIP-190 and BubR1 both localise to primary constrictions of all chromosomes in the absence of microtubules. Note that CLIP-190 localises to the outer regions of kinetochores relative to BubR1. (C) Localisation of CLIP-190 to unattached kinetochores. Chromosomes were visualised with an antibody against phosphorylated histone H3. Only one of the sister kinetochores, the one facing away from the pole, has CLIP-190. Bars, 10 µm.