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Fig. 2. Constitutively active CamKII{alpha} and PKC{alpha} constructs. (A,B) Schematic of wild-type CamKII{alpha} (A) and PKC{alpha} (B). Constitutively active (CA) mutants of CamKII and PKC were fused C-terminally to GFP. (C) CA-CamKII::GFP; deletion of residues 291-478. (D) CA-E25PKC::GFP; substitution of alanine for glutamic acid at position 25 and CA-{Delta}22-28PKC::GFP; deletion of residues 22-28.