Fig. 3. Constitutively active CamKII
, but not PKC
, induces completion of meiosis. (A) CA-CamKII, but not CA-E25PKC or CA-
22-28PKC, was effective at inducing exit from meiotic arrest. The amount of cRNA injected is plotted against the percentage activation rate, as assessed at 8 hours by the formation of pronuclei. The number of eggs used is indicated in parenthesis. (B) The time course of meiotic progression with CA-CamKII is similar to that observed with sperm, with second polar body extrusion (PB2; indicated by arrow) at about 1.5 hours and pronucleus formation (PN; arrowhead) at about 5.5 hours. Scale bar: 20 µm.