Fig. 3. Proteins co-immunoprecipitating with NcadER activated or not by 40HT. (A) Immunoblot analysis of N-cadherin immunoprecipitates from LN cells and LNER cells with or without 4OHT treatment. LNER cells without (-) or with (+) 100 nM 4OHT overnight were extracted with 0.5% NP40 and the NcadER immunoprecipitated with anti-N-cadherin using identical conditions. N-cadherin was immunoprecipitated from LN cells as a positive control. The immunoprecipitates were resolved by SDS-PAGE, transblotted to nitrocellulose and immunoblotted with antibodies to N-cadherin, ß-catenin, -catenin, actin and p120^ctn. (B) Quantification of N-cadherin co-immunoprecipitating proteins normalized to N-cadherin. Means of two separate experiments are shown. (C) N-cadherin immunoprecipitation of ³⁵S-radiolabeled LNER cells, treated or not with 4OHT, analyzed by SDS-PAGE and autoradiography. The control lane was processed using hybridoma supernatant without