Fig. 3. Overexpression of ChREBP did not affect glucose-stimulated insulin secretion or cell growth. (A) ChREBP^*16 cells were cultured with (+Dox) or without (-Dox) 500 ng/ml doxycycline in standard medium (11.2 mM glucose) for 24 hours and then equilibrated in 2.5 mM glucose medium for a further 24 hours. Insulin release from ChREBP^*16 cells in KRBH buffer containing the indicated concentrations of glucose was determined by radio-immunoassay and expressed as a percentage of cellular insulin content. Data represent six independent experiments. (B) A WST-1 assay, presented as optical density at 440 nm, showed that ChREBP^*16 cell growth/survival was not affected by 48 hours treatment with 500 ng/ml doxycycline.