Fig. 6. Effects of DN-SREBP-1c on gene expression patterns induced by chronic high-glucose in INS-1 cells. DN-SREBP-1c^*23 cells were cultured in either standard (11.2 mM; -) or 30 mM (+) glucose medium for 48 hours, in the presence (+Dox) or absence (-Dox) of 500 ng/ml doxycycline. The gene expression pattern in these cells was quantitatively evaluated by northern blotting. 20 µg total RNA samples were analysed by hybridising with the indicated cDNA probes. The experiments were repeated twice with similar results.