Fig. 1. (A) Western blot analysis of frataxin and VDAC-1 expression in Caco-2 cells extracts at different stages of culture. (B) Relative densitometric analysis of mature frataxin and VDAC-1 expression was normalised to tubulin using NIH Image Software. Data is expressed as fold change over control levels at day 4. (C) D-loop and Cytochrome-b mtDNA expression analysis in Caco-2 cell after the indicated days of culture. Results are expressed as fold change over control levels at day 4. Experiments were carried out three times; results presented are taken from one representative experiment.