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Fig. 5. Western blot analysis of neural and non-neural markers in hMSCs. (A) Neural marker expression in hMSCs cultured in standard growth medium with 10% FBS (Lane 3), in serum-free medium (Lane 4) and with DMSO/BHA for 6 hours (Lane 5) or 48 hours (Lane 6). Protein extracts from human foetal brain (Lane 1) and HEK293 (Lane 2) served as positive and negative controls, respectively. (B) Non-neural marker expression in hMSCs cultured in standard growth medium with 10% FBS (Lane 1), in serum-free medium (Lane 2) and with DMSO/BHA for 6 hours (Lane 3) or 48 hours (Lane 4). ß-actin was used as a loading control (lower panels in A and B).