Fig. 1. Cell-free interaction of Tat with _vß₃ integrin. (A) Aliquots of purified human _vß₃ were incubated on plastic coated with BSA, FN, GST or GST-Tat. At the end of incubation, plastic-bound proteins were extracted and analysed by western blotting with anti-ß₃ antibodies. (B) Purified _vß₃ was injected at (from top to bottom) 100, 50, 25, 18, 15, 10, and 5 nM for 4 minutes over a BIAcore sensorchip coated with GST-Tat. (C) _vß₃ (100 nM) or the buffer alone were injected over BIAcore sensorchips coated with GST-Tat or BSA. In all the experiments the responses (in RU) were recorded as a function of time.