Fig. 3. Confocal images of permeabilized cells that were unstimulated (-EGF) or stimulated with EGF (+ EGF) and co-stained with antibodies against EGFR (green channel) and to CBL (red channel). Green and red channels were merged after both fluorescent signals were adjusted to similar levels. The yellow color indicates the overlap of red and green fluorescence. Areas corresponding to the enlarged insets shown on the extreme right are boxed in all of the panels. Some intracellular vesicles co-stained for EGFR and CBL are highlighted with arrows. Bar, 10 µm.