Fig. 5. (A,B) Cells infected with E3-13.7-positive (+) or E3-13.7-negative (-) adenoviruses were labeled with a mixture of [³⁵S]cysteine and [³⁵S]methionine for 3 hours, and cell lysates were immunoprecipitated with antibodies against E1A (A) or E3-13.7 (B) early adenovirus proteins. (C,D) Adenovirus-infected cells were pulse-labeled for 15 minutes at 16 hours post-infection and were then switched to chase-medium for up to 3 hours (C) or 6 hours (D). Cell lysates were immunoprecipitated with an anti-EGFR antibody. Immunoprecipitates in panels A-D were resolved by SDS-PAGE for autoradiographic detection of radiolabeled proteins. Molecular mass standards: 66,200 (BSA); 45,000 (ovalbumin); 14,400 (lysosyme). (E) Confocal images of permeabilized cells that had been infected with E3-13.7-positive (+ E3-13.7) or E3-13.7-negative (-E3-13.7) adenoviruses and stained with an EGFR-specific antibody. Pos, positive; neg, negative. Bar, 10 µm.