Fig. 2. Differential distribution of the integrin 5 chain and 12G10 reactive ß1 chains on the cell surface. K562 cells were incubated with B3B11 antibody (A-C) or 12G10 (D-F), washed, and a Cy3 (red)-conjugated anti-mouse immunoglobulin second antibody was applied to trigger aggregation of the antibody-reactive ß1 species. The cells were fixed and stained with a polyclonal anti-5, followed by Oregon Green-conjugated second antibody (A-F). A representative double stained cell was selected and photographed. (A) B3B11, (B) 5, (C) overlay of A and B. (D) 12G10, (E) 5, (F) overlay of D and E. (G-I) 5 integrins of K562 cells were cross-linked with polyclonal anti-5 and Oregon Green 488-conjugated secondary antibody. The cells were fixed and stained with 12G10 plus Cy3-conjugated second antibody. (G) 12G10, (H) 5, (I) overlay of G and I.