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Fig. 4. Arl1(Q71L) suppresses redistribution of golgin-97 induced by ARFRP1(T31N) expression. HeLa cells co-expressing a combination of either HA-tagged Arl1(WT) (A,B) or Arl1(Q79L) (C) and either FLAG-tagged ARFRP1(Q79L) (A) or ARFRP1(T31N) (B,C) were triple-stained for HA (middle column), FLAG (left column) and golgin-97 (right column) followed by Alexa Fluor 488-conjugated anti-rat IgG, Cy3-conjugated anti-mouse IgG and Cy5-conjugated anti-rabbit IgG. Bar, 20 µm.