Fig. 1. Illustration of hFis1 constructs used in this study. All hFis1 constructs were tagged with the Myc-epitope at the N-terminus. The hFis1 transmembrane domain spanning the mitochondrial outer membrane is depicted in black near the C-terminal end. Wild-type hFis1 (hFis1-WT) contains six -helices (1-6) in its cytosolic domain. Helices 2-3 and 4-5 form the two TPR motifs, TPR1 and TPR2, respectively.