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Fig. 2. Enhanced accumulation of WRN by pre-treatment of cells with BrdU. (A) Accumulation of GFP-tagged WRN indicated by yellow arrows after higher dose irradiation with the 365 nm laser in HeLa cells with or without pre-treatment with BrdU. (B) Accumulation of GFP-tagged WRN after 10 and 100 scans with the 405 nm laser with or without pre-treatment with BrdU in HeLa cells. (C) Immunochemical detection of {gamma}H2AX after laser irradiation in HeLa cells with or without pre-treatment with BrdU. The number of scans with the 405 nm laser are indicated in yellow. (D) Colocalization of GFP-tagged WRN and {gamma}H2AX at irradiated sites in HeLa cells after 100 scans with the 405 nm laser. (E) Immunochemical detection of endogenous WRN after 405 nm laser irradiation with or without pre-treatment with BrdU in HeLa cells. The number of scans with the 405 nm laser are indicated in yellow. Arrows indicate the sites of irradiation.