Fig. 6. WRN accumulates at double-strand breaks via its HRDC domain in vivo. (A) Domains and mutations introduced in GFP-WRN. (B) Results of accumulation of the mutants of GFP-WRN at double-strand breaks. + means positive and – means negative accumulation. (C) Accumulation kinetics of GFP-tagged full-length WRN (WRN) and the HRDC domain (HRDC) after laser irradiation of HeLa cells. Standard deviations derived from at least three independent data are indicated.