Fig. 8. Immunohistochemistry for c-Kit and SUR. Smooth muscle layer (including the myenteric plexus) of ileum was double-labelled with anti-c-Kit antibody (A: ACK2 with Alexa Fluor 488, red) and anti-SUR1 antibody (B: C16 with Alexa Fluor 594, green); C shows a merged image. Note that network-forming cells were stained yellow, indicating that ICCs in the ileum express SUR1. Scale bar: 20 µm. (D) An enlarged view of the boxed region in C. A single cell with both anti-c-Kit and anti-SUR1 immunoreactivity is indicated by a dotted lines.