Fig. 8. Acid-dependent sequestration of PGI/AMF by FN stimulates cell motility. Confluent monolayers of FN^+/– (blue bars) and FN^–/– (green bars) cells were grown for 1 day in regular medium, wounded by scraping and cell migration from the wound measured after 14 hours. The scraped monolayers were treated with 25 µg ml^–1 PGI/AMF for the complete 14 hour period or treated for 30 minutes with the same concentration of PGI/AMF at pH 5 or 7.5 and then rinsed and incubated in regular medium for the remainder of the 14 hour period. Alternatively, cells were pre-incubated with 10 µg ml^–1 HS for 60 minutes and then incubated with HS or HS plus PGI/AMF for 30 minutes at pH 5 prior to rinsing. The data was normalized to the migration of FN+/–cells in the absence of PGI/AMF or HS (±s.e.m.; n=3).