Fig. 1. The scz6 mutation, a mutant allele of the PKC1 gene, suppresses various phenotypes of the zds1 strain. (A) Effect of scz6/pkc1-834 and stt1-1, mutant alleles of PKC1, on the growth of the zds1 mutant strain on solid medium. Wild-type (DHT22-1b), zds1 (YAT1), zds1 scz6/pkc1-834 (YMM4020), scz6/pkc1-834 (YMM28), zds1 stt1-1 (YMM134), and stt1-1 (YMM114) cells were spotted on YPD plates and grown at 25°C (2 days), or at 33°C or 37°C with or without 300 mM CaCl₂ (2 days). (B,C) Cell morphology (B) and flow cytometry analysis of PI-stained cells (C) of various strains after 6 hours incubation with 100 mM CaCl₂ at 25°C (1C, haploid; 2C, diploid).