Fig. 7. pkc1-834 and stt1-1 are not required for the regulation of Hsl1p abundance. Comparison of Hsl1p abundance in various strains. Wild-type (YMM53), pkc1-834 (YMM143), stt1-1 (YMM144) and mpk1 (YMM70) strains with a chromosomally integrated construct encoding genomic copies of 3xHA-Hsl1p were grown in YPD at 25°C until early log phase. CaCl₂ was added to the cell cultures at the indicated time, to a final concentration of 100 mM, and samples were taken for western blot analysis.