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Fig. 8. Rho1p GTPase is involved in the Ca2+-induced polarized bud growth. Effect of several rho1 mutant alleles on the cell morphology of the zds1{Delta} mutant strain. Wild-type (YOC729), zds1{Delta} (YMM230), zds1{Delta} rho1-2 (YMM232), zds1{Delta} rho1-3 (YMM147), zds1{Delta} rho1-4 (YMM148) and zds1{Delta} rho1-5 (YMM149) cells were used. Cell morphology after 6 hours of incubation with 300 mM CaCl2 at 25°C was examined.