Fig. 8. Equatorial endocytosis is dependent upon exocyst function. (A) Equatorial endocytosis was scored in dividing cells in the presence of BFA using ethanol (Eth) as a control and in sec8-1 cells at the permissive (25°C) and restrictive (36°C) temperatures. (B) Sec8-GFP vesicles and FM4-64-labelled vesicles are largely distinct although some exchange may occur between these compartments. Bar, 10 µm.