Fig. 4. Sorting of ClC-2 Cl^– channel in MDCK cells and Caco-2 cells. MDCK cells (a and b) were grown to confluence upon glass coverslips (a) or Transwell filters (b), transfected with ClC-2-GFP and observed after 24 hours. Specimens were analyzed by confocal microscopy. Representative X-Y and X-Z sections are shown. (c) Same MDCK monolayer as in (a), but stained with an anti-ZO-1 antibody. (d) MDCK monolayer grown on glass coverslip and transfected with c-myc-tagged hSlo K⁺ revealed with anti-c-myc antibody. (e,f) Experiments as in (a) and (b) but performed upon monolayers of Caco-2 cells. Arrows mark the filter position in (b) and (f). Bar: 10 µm, applies to all panels.