Fig. 5. HCR RNAi affects EGFP-RPB3 subcellular localization. (A) Western blot analysis of cycling (GM) myogenic C2C7 cells transfected with pSuper RNAi empty vector and with increasing DNA amounts of pSuper-HCR interference vector. (B) Fluorescence of EGFP-RPB3 fusion protein co-expressed in cycling (GM) myogenic C2C7 cells, either with empty pSuper vector (top) or with pSuper-HCR interference vector (bottom). All nuclei are Hoechst stained.