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Fig. 10. E4-ORF1 causes cell polarity defects in MDCK cells. Shown are cysts derived from MDCK-IIIA cells or MDCK-E4-ORF1 cells co-immunostained with gp135 and E-cadherin antibodies. Representative cysts from independent experiments 1 and 2 were photographed at 7 days (MDCK-IIIA cells) or 10 days and 14 days (MDCK-E4-ORF1 cells) post-suspension. Scale bars are 10 µm (experiment 1) and 20 µm (experiment 2).