Fig. 2. Expression of PaxB during development. (A) PaxB expression during development as detected by western blot with a Dictyostelium-specific affinity purified anti-peptide antibody. Maximal expression is reached at 12-15 hours of development. The experiment shown is representative for results obtained in four independent time course experiments. (B) Expression of a paxB/lacZ expression construct showing expression of paxB during all stages of development. Note and the increased levels of expression in the tip of the slug and the upper and lower cup of culminates. (C) Expression of a C terminal PaxB-GFP knockin construct again showing expression from the vegetative stage onwards and increased expression in the slug and culminate tip.