Fig. 3. Cellular localisation of paxB in a paxB-gfp knockin mutant. (A,B) Localisation of paxillin in a vegetative cell at 0 and 120 seconds. Note the localisation of PaxB in small spots at randomly distributed sites where the cell is in close contact with the substrate (see supplementary material Movie 1). (C,D) Cumulative PaxB distribution of the cell shown in A,B for 0-60 seconds (C) and 60-120 seconds (D). The cumulative distribution shows that many of the spots are stationary and also show more clearly the localisation of PaxB in the tips of the filopodia. Scalebar (A): 10 µm. (E) Localisation in retraction fibres at the back of cells in migrating slugs. (F) Localisation of PaxB in small focal adhesion spots localised at the contact area between the slime sheath and the substrate in the outermost cells of a slug. The slug shown in images E,F is migrating from left to right. These structures are also found in cells deeper in the slug as can be seen in the supplementary information provided (see supplementary material Movie 2). Scale bar in F, 10 µm. (G) Distribution of cell-cell contacts in an early culminant. These contacts are distributed around the cell (marked with arrows) and are most obviously detected in the small epithelial layer of cells surrounding the spore mass in the forming fruiting body as well as in the cells in the upper cup and lower cup (see supplementary material Movie 3). (H) Another culminant showing the presence of PaxB enriched cell-cell contacts (arrows) in the back of an early culminant and (I) a higher magnification image of a central part of the image shown in H. (J) Section through the forming stalk of an early culminant showing high levels of PaxB localisation at the contact area between cells and the stalk sheath. Scale bar, 40 µm. (K) Higher magnification of a maximum projection of a stack of 10 adjacent images of the cells in contact with the stalk tube showing the presence of special PaxB-rich areas between the cells contacting the stalk sheath. Scale bar, 10 µm.