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Fig. 10. Clathrin adaptors on the plasma membrane and TGN are altered in cells transfected with Hsp70(K71E). (A) TIR-FM images of GFP-AP2 (a,b) and GFP-epsin (c,d) in cells cotransfected with either Hsp70 (a,c) or Hsp70(K71E) (b,d). (B) Confocal images of cells transfected with Hsp70(K71E) stained with flag antibodies (a,c,e) and TGN-associated adaptors: AP1 (b), GGA3 (d) and GAK (f).