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Fig. 3. GAK depletion alters distribution of CCPs on plasma membrane. Immunostained clathrin on the plasma membrane was imaged using confocal microscopy in mock-depleted (a) and GAK-depleted (b) cells. Transfected GFP-clathrin on the plasma membrane was imaged using confocal microscopy in mock-depleted (c) and GAK-depleted (d) cells. Transfected GFP-clathrin on the plasma membrane was imaged using TIR-FM in mock-depleted (e) and GAK-depleted (f) cells.