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Fig. 6. Clathrin exchange in GAK-depleted cells. (A) The exchange of GFP-clathrin on CCPs was determined in control (open circles), mock-depleted (solid circles) and GAK-depleted (solid triangles) HeLa cells by measuring the fluorescence recovery after photobleaching. (B) The exchange of cytosolic clathrin in GAK-depleted cells was measured in the presence (open triangles) and absence (solid triangles) of BFA. The inset shows the time course of recovery of cytosolic clathrin measured after treating mock-depleted cells with BFA (open circles). For comparison, the dashed line shows the rate of recovery of the cytosolic clathrin in GAK-depleted cells.