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Fig. 7. Colocalization of GFP-clathrin clusters in GAK-depleted cells with transferrin and EEA1. Confocal images of GFP-clathrin (a,d) and either transferrin (b) or immunostained EEA1 (e) in GAK-depleted cells. The cells internalized Alexa Fluor 546-transferrin for 30 minutes. (c,f) The merged images showing the overlaps.