Fig. 10. Electrophoretic mobility shift assays (EMSAs) for IRPs in SW1088 cells in response to iron exposure (FAC), iron chelation (DFO), calcium chelation (BAPTA), and BAPTA concomitant with FAC (B-FAC) or DFO (B-DFO). IRP/IRE interaction in SW1088 cells decreases upon FAC treatment with or without concomitant addition of the calcium chelator, BAPTA in both the cytosolic and membrane fractions. The IRP/IRE interaction increases upon treatment with DFO alone, BAPTA alone or DFO/BAPTA together in both the cytosolic and membrane fractions. IRP localization to the membrane fraction occurs with iron and calcium chelation. ^*P<0.05.