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Fig. 1. Schematic diagram of focal labeling and observation of initial diffusion (FLOID). (a) The cell body is lightly touched by a micro-needle containing carbocyanine dye. (b) Epi-illumination shows dye spreading in both dorsal and ventral cell membranes. The shortest diffusion path from the labeling point to the leading edge is via the dorsal membrane. (C) TIRF illumination shows only the ventral cell membrane.