Fig. 3. Keratocytes stained with Alexa-488 phalloidin to reveal F-actin distribution after (a) no treatment, (b) extraction of membrane cholesterol with CD, (c) treatment with CA to stop motility, and (d) CA/CB treatment to depolymerise the actin meshwork. The fluorescence intensity of phalloidin stain was reduced by 25% in CD-treated cells compared with controls. Fluorescence intensities cannot be compared directly in these images because they are displayed on different contrast scales. Bars, 5 µm.