Fig. 5. Acid secretory response of parietal cells infected with control rAd and with CFP-ezrin constructs expressing WT and the T567A and T567D mutants. The aminopyrine (AP) uptake was measured for the various cultures of parietal cells in the presence of the presence of the H2-receptor blocker cimetidine (Cim), maximally stimulated by histamine plus IBMX (His+IBMX), and stimulated in the presence of the H,K-ATPase blocking drug SCH-28080 (His+IBMX+SCH). The data were collected from assays on six separate culture preparations, and normalized within each experimental run by setting the AP uptake of His+IBMX for control virus at 100% and shown as mean±s.e.m. All preparations showed highly significant AP uptakes in response to stimulation by His+IBMX. Statistical analysis (t-test) of the maximally stimulated AP uptake revealed that P>0.05 between control virus and ezrin-WT; P=0.05 between ezrin-WT and T567A; and P=0.004 between ezrin-WT and T567D.