Fig. 6. Effect of Met-AEA on spontaneous and zona pellucida (ZP)-induced acrosome reaction (AR) detected by FITC-PSA staining. (A) Percentage of AR in spermatozoa capacitated in vitro for 4 hours with 1 µM Met-AEA, 0.1 µM SR141716 or both. (B) Spontaneous and ZP-induced AR recorded in spermatozoa incubated in the absence or presence of 1 µM Met-AEA, 1 mM 8Br-cAMP or both. In A and B the maximum standard deviation was ±1.5 and ±2.0, respectively; ^*P<0.01 versus CTR, ^**P<0.01 versus Met-AEA for ZP-induced AR. Samples were not statistically different (P>0.05) for spontaneous AR.