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Fig. 4. FRAP experiments at FAs uncover regulation of FAK/YCam molecular dynamics by phosphorylation. (A) U87 cells expressing FAK/YCam (left) or Y397F-FAK/YCam (right) were imaged before bleaching of FA-containing ROIs (white squares). Scale bar: 10 µm. The time lapse sequences (in seconds) below show recovery after bleaching of corresponding FAs for FAK/YCam (upper row) and Y397F-FAK/YCam (lower row). Bpb, before photobleaching; apb, immediately after photobleaching. Scale bar: 2 µm. (B) Kinetics of recovery of FAK/YCam (-µ-) and Y397F-FAK/YCam (-{lambda}-) in cytosolic (top left) and FA compartments (top right) after bleaching. The fluorescence intensity in the bleached region was measured and expressed as the relative recovery. At FAs (bottom right), note the significantly shorter recovery half-time for Y397F-FAK/YCam compared to FAK/YCam.