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Fig. 7. Analysis of the expression of FAK/YCam or Y397F-FAK/YCam and paxillin in U87 astrocytoma cells before and after FA turnover induced by nocodazole wash-out. FAK/YCam- and Y397F-FAK/YCam-transfected cells plated for 2 days on Matrigel were fixed before addition of nocodazole (0'), or 60 (60') minutes after nocodazole wash-out, permeabilized and paxillin was revealed by immunocytochemistry. (A) Before addition of nocodazole, FAK/YCam and Y397F-FAK/YCam colocalized with paxillin. (B) Quantification, using Pearson's coefficient, reveals no difference in colocalisation of YCam and paxillin before addition of nocodazole or after FA disassembly induced by nocodazole wash-out in FAK/YCam cells and Y397F-FAK/YCam cells. Scale bar: 10 µm.