Fig. 8. GFP-ubiquilin is localized to aggresomes upon proteasome inhibition. HeLa cells transiently transfected with the GFP-ubiquilin construct (a-f) or with HA-ubiquilin and the GFP-CFTR constructs (g,h) were mock treated (0.1% DMSO) (a,c,d) or incubated overnight with 5 µM MG132 (b,e-h). Cells were fixed, permeabilized and processed for fluorescence microscopy using the FK2 anti-ubiquitin antibody (c-f) or the anti-HA antibody (g-h). The first antibodies were revealed by Cy3-labelled goat anti-mouse immunoglobulin secondary antibodies. (a-c,e,h) Green fluorescence emitted by GFP. (d,f,g) Red fluorescence emitted by Cy3.