Fig. 2. PLC activity is maintained by Ca²⁺ influx through store-operated channels. Evanescent wave microscopy recording of PH_PLC-GFP-expressing MIN6 cells during sustained PLC activity triggered by 100 µM carbachol. (A) Effect of 250 µM diazoxide. (B) Effect of 10 µM nifedipine and 100 µM 2-APB. (C) Effect of 50 µM SKF96365. (D) Effect of 100 µM carbachol and 100 µM 2-APB in cells expressing membrane-targeted GFP. (E) Mean±s.e.m. for the effects of diazoxide, nifedipine, 2-APB and SKF96365 on the steady-state PH_PLC-GFP fluorescence during stimulation with carbachol. ^*P<0.01; ^**P<0.001. (F) Effect of carbachol when added after 100 µM 2-APB. (G) Effect of store-operated Ca²⁺ influx triggered by 100 µM CPA on PH_PLC-GFP fluorescence.