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Fig. 3. SNAKA51 only colocalizes with fibronectin fibers and primed ß1 integrin in fibrillar adhesions. Human fibroblasts were fixed and stained using indirect immunofluorescence. (a) {alpha}5 integrin [SNAKA51 (left), SNAKA52 (middle), mAb11 (right), all green], fibronectin (blue) and {alpha}V integrin (red) as a focal adhesion marker (L230). (b) ß1 integrin in a primed conformation (9EG7) (red) and {alpha}5 integrin (SNAKA51, green) (top). Total ß1 integrin (mAb11 red) and {alpha}5 integrin (SNAKA51, green) (middle). ß1 integrin in a primed conformation (9EG7, red) and total ß1 integrin (mAb11, green) (bottom). Bar 20 µm, inset bar 5 µm.