Fig. 5. Effects of Wnt3a on nuclear accumulation of the p-ERK and ß-catenin proteins in NIH3T3 cells. NIH3T3 cells were grown in DMEM and treated with 150 ng/ml recombinant Wnt3a. Cells were subjected to immunocytochemical analysis at 0, 30 minutes and 8 hours after Wnt3a treatment. Cells were then treated with anti-p-ERK and anti-ß-catenin antibodies. p-ERK proteins were detected as a green color using Cy^TM2-conjugated goat anti-mouse IgG, ß-catenin proteins were detected as a red color using anti-rabbit-Rhodamine Red ^TM-X-conjugated secondary antibody. Cell nuclei were stained with DAPI. Bar, 10 µm.