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Fig. 3. Effect of CLPT1 and CLPT1(N123I) on pectinase secretion. The mycelium was grown for 4 days on glucose medium before being transferred to pectin medium. Extracellular polygalacturonase activity produced by the different strains was measured using polygalacturonic acid as substrate. The data are the mean of three independent experiments. {square}, wild-type, {triangleup}, transgenic control strain (empty vector); {diamond}, CLPT1.4; x, N123I.6; , N123I. 9.