Fig. 4. Cell wall thickenings induced by CLPT1(N123I). Confocal laser scanning microscopy of Congo Red-stained hyphae from wild type (WT), CLPT1- (CLPT1.9) or CLPT1(N123I) (N123I.6) expressing strains grown on a cellophane membrane placed on pectin medium. Each image is a projection of 10-15 confocal planes acquired in the z dimension. Note the accumulation of numerous Congo Red stained spots (white arrows) along the hyphal cell wall of N123I.6. Bars, 5 µm.