Fig. 6. Development of infection structures and pathogenicity of transgenic strains expressing CLPT1(N123I). (A) Conidia (Co) of C. lindemuthianum wild-type (WT) and CLPT1(N123I) strains (N123I.6 and N123I.9) germinating on glass slides to form appressoria (Ap). Note the abnormal development of appressoria in the N123I strains. (B) Bean leaves were inoculated with a suspension of conidia from the wild-type strain (WT) or from the CLPT1(N123I) strains (N123I.6; N123I.9). (C) Details of anthracnose lesions produced by the different strains. (D) Microscopic examination of infected bean tissues. The wild-type strain (WT) develops an appressorium (Ap) that penetrates a hypocotyl epidermal cell and differentiates an infection vesicle (IV) 24 hours after inoculation. The N123I.6 conidia germinate, fail to penetrate host cells and grow saprophytically on the surface of the plant tissue.