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Fig. 3. Overexpressed but not internalized S100A1 colocalizes with SERCA2a at the SR. Immunolocalization of S100A1 and SERCA2a. (A) Endogenous S100A1 labeled with a monoclonal anti-S100A1/Cy5-anti-mouse antibody in control cells. (A') Overexpressed S100A1 detected with a polyclonal anti-human S100A1/Cy5-anti-rabbit antibody in AdS100A1-transduced NVCMs. (A") Internalized Rh-S100A1. (B) Immunolabeling of SERCA2a in control, (B') AdS100A1-infected, (B") and Rh-S100A1-treated NVCMs. (C) Overlay of A and B, and C', A' and B' depicts colocalization of endogenous and overexpressed S100A1 with SERCA2a (violet). (C") Internalized Rh-S100A1 does not colocalize. Bar, 10 µm (C') and 5 µm (C,C").