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Fig. 5. Homologous recombination is reduced in mcp6{Delta} cells but ectopic recombination is increased compared with wild-type (WT) cells. The chromosomal positions of the loci and centromeres are illustrated in the insets. (A) Intergenic recombination (crossing over) showing the intervals between leu1 and his2 (left), lys3 and cdc12 (middle) and the primary tetrad (right). Only those tetrads that generated four viable spores were used to calculate the genetic distances (cM). The strains examined for leu1-his2 crossing were WT (TT8-1 x NP32-2A) and mcp6{Delta} (TT398 x TT399), whereas the strains used for the lys3-cdc12 crossing were WT (TT8-1 x TT231-1) and mcp6{Delta} (TT399 x TT411). The data shown are the average values calculated from at least three independent assays (at least 40 tetrads were dissected per assay). (B) Intragenic recombination. The strains examined were WT (MS105-1B x MS111w1) and mcp6{Delta} (TT400 x TT401). The average values were calculated from at least three independent assays. (C) Ectopic intragenic recombination. The strains crossed were WT (MS105-1B x GP1123) and mcp6{Delta} (TT400 x TT1014). The average values were calculated from at least three independent assays. Standard deviations are indicated as error bars. (D) Spores of mcp6{Delta} cells are almost normal as judged by the frequency of abnormal ascospores. The haploid parental strains were mated and sporulated on ME plate. After overnight culture, the cells were fixed with 70% ethanol for staining with Hoechst 33342. At least 200 cells were counted. (E) Spore viability of WT (TP4-5A x TP4-1D) and mcp6{Delta} (TT397-5A x TT397-1D) cells. Random spore analysis was performed.