Fig. 7. The subcellular localization of GFP-tagged SPB components at the horsetail phase is normal in mcp6 cells. The h⁹⁰ strains that express Sad1-DsRed (WT, CRL790; mcp6, ST148) (A), Spo15-GFP (WT, ST176; mcp6, ST171-1) (B) or Kms1-GFP (WT, ST191-1; mcp6, ST172-1) (C) fusion proteins were induced to enter meiosis by nitrogen starvation. After 6 hours, the cells were collected and observed under a fluorescence microscope. Typical images are shown. (D) The proportions of the cell population in which Sad1-DsRed localized to the leading edge of the nucleus with a single dot, as depicted in the inset. (E) The proportions of the cell population in which Spo15-GFP and Sad1-DsRed colocalized to the leading edge of the nucleus with a single dot, as depicted in the inset. (F) The proportions of the cell population in which Kms1-GFP and Sad1-DsRed colocalized to the leading edge of the nucleus, as depicted in the inset. Green, GFP; red, Sad1-DsRed; blue, Hoechst 33342. The dotted line depicts the contour of the cell. Bar, 5 µm.