Fig. 8. GFP-tagged telomere components and -tubulin localize normally in mcp6 cells. The h⁹⁰ strains that express Taz1-GFP (WT, ST178; mcp6, ST173) (A), Swi6-GFP (WT, ST179-1; mcp6, ST174) (B) or -tubulin-GFP (WT, YY105; mcp6, ST146) (C) were induced to enter meiosis by nitrogen starvation. After 6 hours, the cells were collected and observed under a fluorescence microscope. Images shown in (C) were obtained by immunofluorescence. Typical images are shown. (D) The proportions of the cell population in which Taz1-GFP and Sad1-DsRed colocalized to the leading edge of the nucleus with a single dot, as depicted in the inset. (E) The proportions of the cell population in which Swi6-GFP and Sad1-DsRed colocalized to the leading edge (L.E.) of the nucleus with extra dots of Swi6-GFP in the nucleus, as depicted in the insets. (F) The proportions of the cell population that display normal or abnormal (i) and (ii) tubulin positioning as depicted on the right. Green, GFP; red, Sad1-DsRed (A,B) or Sad1 (C); blue, Hoechst 33342. The dotted line indicates the contour of the cell. Bar, 5 µm.