Fig. 3. Immunolocalisation of ASY1 and AtSCC3 in wild-type and Atscc3-1 mutant cells. (A-AD) Male meiotic cells. (AE-AG) A wild-type vegetative cell, easily distinguishable from meiocytes based on its size and small nucleolus. (A,B,C) Early interphase meiocyte, probably in G1 as ASY1 labelling is very faint. (D,E,F) Interphase meiocyte, probably in G2 according to ASY1 staining, which is strong. (G,H,I) Leptotene; (J,K,L) zygotene; (M,N,O) pachytene; (P,Q,R) diakinesis; (S-X) metaphase I; (Y-AA) zygotene; (AB-AD) pachytene. Arrows in S and V indicate centromeres, and arrows in W and X indicate the AtSCC3 staining corresponding to chromosome arms. For each cell, several stains are shown: anti-ASY1 (in red), anti-SCC3 (in green), and in blue, the DAPI staining of chromatin (DAPI). For diakinesis and metaphase I cells, no ASY1 staining is shown, but an overlay of DAPI and AtSCC3 staining is shown (merge). Bar, 10 µm.